Magnetic Tweezers for the Measurement of Twist and Torque
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چکیده
منابع مشابه
Magnetic Tweezers for the Measurement of Twist and Torque
Single-molecule techniques make it possible to investigate the behavior of individual biological molecules in solution in real time. These techniques include so-called force spectroscopy approaches such as atomic force microscopy, optical tweezers, flow stretching, and magnetic tweezers. Amongst these approaches, magnetic tweezers have distinguished themselves by their ability to apply torque w...
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Cantilevers and optical tweezers are widely used for micromanipulating cells or biomolecules for measuring their mechanical properties. However, they do not allow easy rotary motion and can sometimes damage the handled material. We present here a system of magnetic tweezers that overcomes those drawbacks while retaining most of the previous dynamometers properties. Electromagnets are coupled to...
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The mechanical properties of DNA fundamentally constrain and enable the storage and transmission of genetic information and its use in DNA nanotechnology. Many properties of DNA depend on the ionic environment due to its highly charged backbone. In particular, both theoretical analyses and direct single-molecule experiments have shown its bending stiffness to depend on salt concentration. In co...
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directly monitor changes in the twist of nucleic acids Jan Lipfert, Matthew Wiggin, Jacob W.J. Kerssemakers, Francesco Pedaci & Nynke H. Dekker Nature Communications 2:439 doi: 10.1038/ncomms1450 (2011); Published 23 Aug 2011; Updated 27 May 2015 The published version of this article contains two errors. In equation (18), there is a typographical error in the second term; it should read Rcircle...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2014
ISSN: 1940-087X
DOI: 10.3791/51503